Upper Midwest Environmental Sciences Center
Liquid chromatographic determination of florfenicol in the plasma of multiple species of fish
Vue, C., Schmidt, L. J., Stehly, G. R., and Gingerich, W. H., 2002, Liquid chromatographic determination of florfenicol in the plasma of multiple species of fish: Journal of Chromatography B Analytical Technologies in the Biomedical and Life Sciences, v. 780, no. 1, p. 111-117.
A simple method was developed for determining florfenicol concentration in a small volume (250 µl) of plasma from five phylogenetically diverse species of freshwater fish. Florfenicol was isolated from the plasma matrix through C18 solid-phase extraction and quantified by reversed-phase high-performance liquid chromatography with UV detection. The accuracy (84-104%), precision (%RSD≤8), and sensitivity (quantitation limit <30 ng/ml) of the method indicate its usefulness for conducting pharmacokinetic studies on a variety of freshwater fish.
fish/florfenicol, Salmo salar L, Atlantic salmon, chloramphenicol, thiamphenicol, efficacy